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Bdnf thesis

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Rauskolb, Stefanie. Brain-derived neurotrophic factor : generation and characterization of adult mice lacking BDNF in the adult brain. Downloads: Statistics Overview. Repository Staff Only: item control page.
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Harnessing BDNF: How Exercise Can Improve Brain Function

Bdnf thesis
Bdnf thesis
Bdnf thesis
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"The effects of BDNF Knockdown on Neuroinflammation in the Male and Fem" by Katheryn Burford

I then designed a protocol to detect these miRNAs with Locked Nucleic Acid LNA in situ hybridization in human prefrontal cortex and determine their layer and cellular expression patterns. As an example, miRa-5p was found to be enriched in large pyramidal neurons of layer 3, which was verified using laser capture microdissection of layer 3 pyramidal neurons and quantitative Real Time Polymerase Chain Reaction qRT-PCR following dissection of upper and deeper layers of human PFC. According to my results differences in miR levels in a subset of subjects diagnosed with schizophrenia were found to be associated with disease related changes in BDNF protein levels and deficits in BDNF dependent GABAergic gene expression. In the last part of my work I focused on miRb, another member of the miR family, which I found to be reduced in the prefrontal cortex of female but not male subjects with schizophrenia. More importantly, disease related changes in miRb levels were strongly associated with the age of onset of the disease. Additional experiments in mouse cortex and hippocampus revealed a gender dimorphic expression pattern of this miRNA with higher expression in female brain. Collectively, my results suggest that miRNAs could participate in novel molecular pathways that play an important role during cortical development and maturation and are potentially linked to the pathophysiology of neuropsychiatric disease.
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Brain-derived neurotrophic factor

Timothy C. Neuroblastoma is the most common extra-cranial solid tumor in children, arising from neural crest precursor cells. High expression of TrkA and TrkC receptors have been linked to favorable biological features and high patient survival, while TrkB is expressed in unfavorable, aggressive tumors. Several studies suggest that high levels and activation of TrkB by its ligand brain-derived neurotrophic factor BDNF stimulates tumor cell survival, proliferation, and chemoresistance.
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Assessment of peripheral BNDF levels over 30 days. Brain health, and the benefits of exercise have been linked to the biological signaling molecule called brain-derived neurotrophic factor BDNF. Animal and human studies have provided some support for physical exercise as a mechanism for increasing BDNF levels. However, results have been inconsistent, which may be attributed in part to incomplete information about normal variation in circulating peripheral BDNF levels. This investigation examined capillary-drawn whole blood samples from nine healthy adult participants over 30 days with the goal of documenting variability in resting BDNF levels and changes that may be attributed to physical exercise.
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